ALTERNATE NAMES: None reported
LENGTH: 187 nts
PROCESS: Pseudouridine formation in rRNA
TARGET SITE(S): 25S rRNA: Y2340
GENOMIC ORGANIZATION: Independent gene
GENE DISRUPTION PHENOTYPE: Viable
CORRESPONDENCES IN HUMANS AND PLANTS: Click here and see that yeast and humans have corresponding modifications and guide snoRNAs.
None reported in plants
PHYLOGENETIC CONSERVATION IN FUNGI: Click here to examine conservation of the snoRNA in fungal genomes using BLAST.
RNA SEQUENCE:
1 gggaauauaa uacuaaauac ucuguuauau agaacuuucu acgccuuuuc cucuauggcu 61 augaaagcgu ggacaacgga gaucaaauag accaauuacu gaauauggcu uuugacauau 121 cuucuucuuc augggugagg auuaagcuga uauccugacc caccuaucau uaguccuuca 181 gacauac
YEAST GENOME DATABASE ENTRY:Click here to view the SGD entry for this snoRNA.
REFERENCES: Balakin, A. G., L. Smith, and M. J. Fournier. 1996. The RNA world of the nucleolus: two major families of small RNAs defined by different box elements with related functions. Cell 86:823-834. Wise, J. A., D. Tollervey, D. Maloney, H. Swerdlow, E. J. Dunn, and C. Guthrie. 1983. Yeast contains small nuclear RNAs encoded by single copy genes. Cell 35:743-751. Parker, R., T. Simmons, E. O. Shuster, P. G. Siliciano, and C. Guthrie. 1988. Genetic analysis of small nuclear RNAs in Saccharomyces cerevisiae: viable sextuple mutant. Mol. Cell. Biol. 8:3150-3159. Ganot, P., M. Caizergues-Ferrer, and T. Kiss. 1997. The family of box ACA small nucleolar RNAs is defined by an evolutionarily conserved secondary structure and ubiquitous sequence elements essential for RNA accumulation. Genes Dev. 11:941-956. Lafontaine, D. L., C. Bousquet-Antonelli, Y. Henry, M. Caizergues-Ferrer, and D. Tollervey. 1998. The box H + ACA snoRNAs carry Cbf5p, the putative rRNA pseudouridine synthase. Genes Dev. 12:527-537. Watkins, N. J., A. Gottschalk, G. Neubauer, B. Kastner, P. Fabrizio, M. Mann, and R. Luhrmann. 1998. Cbf5p, a potential pseudouridine synthase, and Nhp2p, a putative RNA-binding protein, are present together with Gar1p in all H BOX/ACA-motif snoRNPs and constitute a common bipartite structure. RNA 4:1549-1568. Henras, A., Y. Henry, C. Bousquet-Antonelli, J. Noaillac-Depeyre, J. P. Gelugne, and M. Caizergues-Ferrer. 1998. Nhp2p and Nop10p are essential for the function of H/ACA snoRNPs. EMBO J. 17:7078-7090.
