snR8

ALTERNATE NAMES: None reported

LENGTH: 189 nts

PROCESS: Pseudouridine formation in rRNA

TARGET SITE(S):
25S rRNA: Y960
25S rRNA: Y986

Guide Domains
  Click number to see the target site in the 2D structure: 25S-960, 25S-986
Click number to see the approximate location of the modification site in the ribosome: 25S-960, 25S-986

GENOMIC ORGANIZATION: Independent gene
 

SGD ORF MAP
ORFmap

GENE DISRUPTION PHENOTYPE: Viable

CORRESPONDENCES IN HUMANS AND PLANTS:
Click here and see that yeast and humans have corresponding modifications and guide snoRNAs.

None reported in plants

PHYLOGENETIC CONSERVATION IN FUNGI:
Click here to examine conservation of the snoRNA in fungal genomes using BLAST.

RNA SEQUENCE:

   1 augauacaua uuaaacaugc cuucuauuca cugugccauc uuacauccgu aggugaucag
  61 aggggaaaag aagagcuaga gcaacuccca cacucaugag cguccucaua cuguucuggg
 121 cgcuucucac aggugcuucu uuuagugucu cccauuggcc cagaucggua cugcgcgagu
 181 gagauaucu
 

YEAST GENOME DATABASE ENTRY:
Click here to view the SGD entry for this snoRNA.

REFERENCES:
Balakin, A. G., L. Smith, and M. J. Fournier. 1996. The RNA world of the nucleolus: two major families of small RNAs defined by different box elements with related functions. Cell 86:823-834.
Wise, J. A., D. Tollervey, D. Maloney, H. Swerdlow, E. J. Dunn, and C. Guthrie. 1983. Yeast contains small nuclear RNAs encoded by single copy genes. Cell 35:743-751.
Parker, R., T. Simmons, E. O. Shuster, P. G. Siliciano, and C. Guthrie. 1988. Genetic analysis of small nuclear RNAs in Saccharomyces cerevisiae: viable sextuple mutant. Mol. Cell. Biol. 8:3150-3159.
Ni, J., A. L. Tien, and M. J. Fournier. 1997. Small nucleolar RNAs direct site-specific synthesis of pseudouridine in ribosomal RNA. Cell 89:565-573.
Ganot, P., M. Caizergues-Ferrer, and T. Kiss. 1997. The family of box ACA small nucleolar RNAs is defined by an evolutionarily conserved secondary structure and ubiquitous sequence elements essential for RNA accumulation. Genes Dev. 11:941-956.
Lafontaine, D. L., C. Bousquet-Antonelli, Y. Henry, M. Caizergues-Ferrer, and D. Tollervey. 1998. The box H + ACA snoRNAs carry Cbf5p, the putative rRNA pseudouridine synthase. Genes Dev. 12:527-537.
Watkins, N. J., A. Gottschalk, G. Neubauer, B. Kastner, P. Fabrizio, M. Mann, and R. Luhrmann. 1998. Cbf5p, a potential pseudouridine synthase, and Nhp2p, a putative RNA-binding protein, are present together with Gar1p in all H BOX/ACA-motif snoRNPs and constitute a common bipartite structure. RNA 4:1549-1568.
Henras, A., Y. Henry, C. Bousquet-Antonelli, J. Noaillac-Depeyre, J. P. Gelugne, and M. Caizergues-Ferrer. 1998. Nhp2p and Nop10p are essential for the function of H/ACA snoRNPs. EMBO J. 17:7078-7090.


Last update: October 12, 2006 5:06 PM.