snR81
ALTERNATE NAMES: None reported
LENGTH: 188 nts
PROCESS: Pseudouridine formation in rRNA and snRNA
TARGET SITE(S):
U2 snRNA : Y 42
25S rRNA : Y 1052
Click number to see the target site in the 2D structure of U2 snRNA: U2-42
Click number to see the to see the target site in the 2D structure of 25S rRNA: 25S-1052
Click number to see the approximate location of the modification site in the ribosome: 25S-1052
GENOMIC ORGANIZATION: Independent gene
SGD ORF MAP
GENE DISRUPTION PHENOTYPE: Viable
CORRESPONDENCES IN HUMANS AND PLANTS: Click here and see that yeast and humans have corresponding modifications and guide snoRNAs.
None reported in plants
PHYLOGENETIC CONSERVATION IN FUNGI:
Schizosaccharomyces pombe has an orthologous snoRNA (although not noted in the paper originally, snR81 is a functional homolog of pombe snR93).
Click here to examine conservation of the snoRNA in fungal genomes using BLAST.
RNA SEQUENCE:
1 agguuaggga cugcaaaaga agcggcgagg cagcccacau caaguggaac uacacagacu 61 uccuugucgc gauacuacgg ucccaagagc aauccuaaca agcaauuaca uauucccccg 121 cugaaccugu acaguccacg gauggugcag aaguuauaug auuuggggga agacgcuuuu 181 ucacaucu
YEAST GENOME DATABASE ENTRY:Click here to view the SGD entry for this snoRNA.
REFERENCES:
Schattner, P., W. A. Decatur, C. A. Davis, M. Ares, Jr., M. J. Fournier, and T. M. Lowe. 2004. Genome-wide searching for pseudouridylation guide snoRNAs: analysis of the Saccharomyces cerevisiae genome. Nucleic Acids Res. 32 :4281-4296.
Torchet, C., G. Badis, F. Devaux, G. Costanzo, M. Werner, and A. Jacquier. 2005. The complete set of H/ACA snoRNAs that guide rRNA pseudouridylations in Saccharomyces cerevisiae. RNA 11 :928-938.
Ma, X., C. Yang, A. Alexandrov, E. J. Grayhack, I. Behm-Ansmant, and Y. T. Yu. 2005. Pseudouridylation of yeast U2 snRNA is catalyzed by either an RNA-guided or RNA-independent mechanism. EMBO J. 24 :2403-2413.