snR49

ALTERNATE NAMES: None reported

LENGTH: 170 nts

PROCESS: Pseudouridine formation in rRNA

TARGET SITE(S):
18S rRNA: Y120 , Y211 , Y302
25S rRNA: Y990

Guide Domains
  Click number to see the target site in the 2D structure: 18S-120, 18S-211, 18S-302, 25S-990
Click number to see the approximate location of the modification site in the ribosome: 18S-120, 18S-302, 25S-990

GENOMIC ORGANIZATION: Independent gene
 

SGD ORF MAP
ORFmap

GENE DISRUPTION PHENOTYPE: Viable

CORRESPONDENCES IN HUMANS AND PLANTS:
Click here and see that yeast and humans have corresponding modifications and guide snoRNAs.

Click here and see that yeast and Arabidopsis have corresponding modifications and guide snoRNAs.

PHYLOGENETIC CONSERVATION IN FUNGI:
Click here to examine conservation of the snoRNA in fungal genomes using BLAST.

RNA SEQUENCE:

  1 augggacuuu aucuccauga cuaugccauu ucuagguagc cuaugguaaa cgaaucccgu
 61 agauuauuca cacguuuguu gucuuuguug acaacaacac agaguaagaa aaagcaaaag
121 auuuaucucu uuuguccauc uuuuuuuuau uuugaaggcg uguaacauuu
 

YEAST GENOME DATABASE ENTRY:
Click here to view the SGD entry for this snoRNA.

REFERENCES:
Balakin, A. G., L. Smith, and M. J. Fournier. 1996. The RNA world of the nucleolus: two major families of small RNAs defined by different box elements with related functions. Cell 86:823-834.
Ganot, P., M. L. Bortolin, and T. Kiss. 1997. Site-specific pseudouridine formation in preribosomal RNA is guided by small nucleolar RNAs. Cell 89:799-809.
Lafontaine, D. L., C. Bousquet-Antonelli, Y. Henry, M. Caizergues-Ferrer, and D. Tollervey. 1998. The box H + ACA snoRNAs carry Cbf5p, the putative rRNA pseudouridine synthase. Genes Dev. 12:527-537.
Watkins, N. J., A. Gottschalk, G. Neubauer, B. Kastner, P. Fabrizio, M. Mann, and R. Luhrmann. 1998. Cbf5p, a potential pseudouridine synthase, and Nhp2p, a putative RNA-binding protein, are present together with Gar1p in all H BOX/ACA-motif snoRNPs and constitute a common bipartite structure. RNA 4:1549-1568.
Henras, A., Y. Henry, C. Bousquet-Antonelli, J. Noaillac-Depeyre, J. P. Gelugne, and M. Caizergues-Ferrer. 1998. Nhp2p and Nop10p are essential for the function of H/ACA snoRNPs. EMBO J. 17:7078-7090.
Schattner, P., W. A. Decatur, C. A. Davis, M. Ares, Jr., M. J. Fournier, and T. M. Lowe. 2004. Genome-wide searching for pseudouridylation guide snoRNAs: analysis of the Saccharomyces cerevisiae genome. Nucleic Acids Res. 32:4281-4296.
Torchet, C., G. Badis, F. Devaux, G. Costanzo, M. Werner, and A. Jacquier. 2005. The complete set of H/ACA snoRNAs that guide rRNA pseudouridylations in Saccharomyces cerevisiae. RNA 11:928-938.
Last update: October 13, 2006 7:07 AM.