ALTERNATE NAMES: None reported
LENGTH: 209 nts
PROCESS: Pseudouridine formation in rRNA
TARGET SITE(S): 5.8S rRNA: Y73 25S rRNA: Y966
GENOMIC ORGANIZATION: Independent gene
GENE DISRUPTION PHENOTYPE: Viable
CORRESPONDENCES IN HUMANS AND PLANTS: Click here and see that yeast and humans have corresponding modifications and guide snoRNAs.
None reported in plants
PHYLOGENETIC CONSERVATION IN FUNGI: Click here to examine conservation of the snoRNA in fungal genomes using BLAST.
RNA SEQUENCE:
1 cacuccuguu ucugccucau gucuuuuaaa acgcuuccac gggacauggg uucuaauuau 61 ggagaagauc aagcuuugaa auggcccguu uacacauuuu gauacaaccg uagacggcgu 121 cucguuucaa agaccgugug guugucuauc aguauauacu cauuguguuu uucaaaaaau 181 cucuggguug uuuagaugcc acuauauuu
YEAST GENOME DATABASE ENTRY:Click here to view the SGD entry for this snoRNA.
REFERENCES: Decatur, W.A., and Schnare, M.N. 2008. Different mechanisms for pseudouridine formation in yeast 5S and 5.8S rRNAs. Mol Cell Biol. 28:3089-100. Epub 2008 Mar 10. Balakin, A. G., L. Smith, and M. J. Fournier. 1996. The RNA world of the nucleolus: two major families of small RNAs defined by different box elements with related functions. Cell 86:823-834. Ganot, P., M. Caizergues-Ferrer, and T. Kiss. 1997. The family of box ACA small nucleolar RNAs is defined by an evolutionarily conserved secondary structure and ubiquitous sequence elements essential for RNA accumulation. Genes Dev. 11:941-956. Henras, A., Y. Henry, C. Bousquet-Antonelli, J. Noaillac-Depeyre, J. P. Gelugne, and M. Caizergues-Ferrer. 1998. Nhp2p and Nop10p are essential for the function of H/ACA snoRNPs. EMBO J. 17:7078-7090. Peattie, D. A. 1979. Direct chemical method for sequencing RNA. Proc. Natl. Acad. Sci. U. S. A. 76:1760-1764. Rubin, G. M. 1973. The nucleotide sequence of Saccharomyces cerevisiae 5.8 S ribosomal ribonucleic acid. J. Biol. Chem. 248:3860-3875. Torchet, C., G. Badis, F. Devaux, G. Costanzo, M. Werner, and A. Jacquier. 2005. The complete set of H/ACA snoRNAs that guide rRNA pseudouridylations in Saccharomyces cerevisiae. RNA 11:928-938. Watkins, N. J., A. Gottschalk, G. Neubauer, B. Kastner, P. Fabrizio, M. Mann, and R. Luhrmann. 1998. Cbf5p, a potential pseudouridine synthase, and Nhp2p, a putative RNA-binding protein, are present together with Gar1p in all H BOX/ACA-motif snoRNPs and constitute a common bipartite structure. RNA 4:1549-1568.
