ALTERNATE NAMES: None reported
LENGTH: 203 nts
PROCESS: Pseudouridine formation in rRNA
TARGET SITE(S): 25S rRNA: Y2826 25S rRNA: Y2880
GENOMIC ORGANIZATION: Independent gene
GENE DISRUPTION PHENOTYPE: Viable
CORRESPONDENCES IN HUMANS AND PLANTS: Click here and see that yeast and humans have corresponding modifications and guide snoRNAs.
Click here and see that yeast and Arabidopsis have corresponding modifications and guide snoRNAs.
PHYLOGENETIC CONSERVATION IN FUNGI: Click here to examine conservation of the snoRNA in fungal genomes using BLAST.
RNA SEQUENCE:
1 gaaucaaaaa uuuauuuuuu acacggaaac gaugccacag uugacugaac cugucuucua 61 acaguaguua auugccagua uuucaaacaa gauuugaaau acgaguuucc cagaauaauu 121 uauuuggaca ggauaggaag uccgauuucu guguugucuc aaacgaggcg auagaauugg 181 gaugucgaag aaagucuaca uua
YEAST GENOME DATABASE ENTRY:Click here to view the SGD entry for this snoRNA.
REFERENCES: Balakin, A. G., L. Smith, and M. J. Fournier. 1996. The RNA world of the nucleolus: two major families of small RNAs defined by different box elements with related functions. Cell 86:823-834. Samarsky, D. A., A. G. Balakin, and M. J. Fournier. 1995. Characterization of three new snRNAs from Saccharomyces cerevisiae: snR34, snR35 and snR36. Nucleic Acids Res. 23:2548-2554. Ni, J., A. L. Tien, and M. J. Fournier. 1997. Small nucleolar RNAs direct site-specific synthesis of pseudouridine in ribosomal RNA. Cell 89:565-573. Ganot, P., M. L. Bortolin, and T. Kiss. 1997. Site-specific pseudouridine formation in preribosomal RNA is guided by small nucleolar RNAs. Cell 89:799-809. Ganot, P., M. Caizergues-Ferrer, and T. Kiss. 1997. The family of box ACA small nucleolar RNAs is defined by an evolutionarily conserved secondary structure and ubiquitous sequence elements essential for RNA accumulation. Genes Dev. 11:941-956. Lafontaine, D. L., C. Bousquet-Antonelli, Y. Henry, M. Caizergues-Ferrer, and D. Tollervey. 1998. The box H + ACA snoRNAs carry Cbf5p, the putative rRNA pseudouridine synthase. Genes Dev. 12:527-537. Watkins, N. J., A. Gottschalk, G. Neubauer, B. Kastner, P. Fabrizio, M. Mann, and R. Luhrmann. 1998. Cbf5p, a potential pseudouridine synthase, and Nhp2p, a putative RNA-binding protein, are present together with Gar1p in all H BOX/ACA-motif snoRNPs and constitute a common bipartite structure. RNA 4:1549-1568. Henras, A., Y. Henry, C. Bousquet-Antonelli, J. Noaillac-Depeyre, J. P. Gelugne, and M. Caizergues-Ferrer. 1998. Nhp2p and Nop10p are essential for the function of H/ACA snoRNPs. EMBO J. 17:7078-7090. King, T. H., B. Liu, R. R. McCully, and M. J. Fournier. 2003. Ribosome structure and activity are altered in cells lacking snoRNPs that form pseudouridines in the peptidyl transferase center. Mol. Cell 11:425-435.
