ALTERNATE NAMES: None reported
LENGTH: 258 nts
PROCESS: Pseudouridine formation in rRNA
TARGET SITE(S): 25S rRNA: Y2416
GENOMIC ORGANIZATION: Independent gene
GENE DISRUPTION PHENOTYPE: Viable
CORRESPONDENCES IN HUMANS AND PLANTS: Click here and see that yeast and humans have corresponding modifications and guide snoRNAs.
None reported in plants
PHYLOGENETIC CONSERVATION IN FUNGI: Click here to examine conservation of the snoRNA in fungal genomes using BLAST.
RNA SEQUENCE:
1 aauaaugucu uuuauaauag acacccagag ucacgauucg ucuugguuuu agagauucug 61 uuucgacagu uuuuuuaggc gguggacgua uauaguuuac uuaaucguuu acuauacggu 121 ggaguacuaa uacuauuuca cugucgugcu cgcgguugau cuuagauaaa uauauuucuu 181 uuucauaaag aaagugagug gaucuucccu ggaaguacga auaauguacu uaugauuaga 241 uucucuucac gcacauac
YEAST GENOME DATABASE ENTRY:Click here to view the SGD entry for this snoRNA.
REFERENCES: Balakin, A. G., L. Smith, and M. J. Fournier. 1996. The RNA world of the nucleolus: two major families of small RNAs defined by different box elements with related functions. Cell 86:823-834. Clark, M. W., M. L. Yip, J. Campbell, and J. Abelson. 1990. SSB-1 of the yeast Saccharomyces cerevisiae is a nucleolar-specific, silver-binding protein that is associated with the snR10 and snR11 small nuclear RNAs. J. Cell Biol. 111:1741-1751. Ganot, P., M. Caizergues-Ferrer, and T. Kiss. 1997. The family of box ACA small nucleolar RNAs is defined by an evolutionarily conserved secondary structure and ubiquitous sequence elements essential for RNA accumulation. Genes Dev. 11:941-956. Ganot, P., M. L. Bortolin, and T. Kiss. 1997. Site-specific pseudouridine formation in preribosomal RNA is guided by small nucleolar RNAs. Cell 89:799-809. Lafontaine, D. L., C. Bousquet-Antonelli, Y. Henry, M. Caizergues-Ferrer, and D. Tollervey. 1998. The box H + ACA snoRNAs carry Cbf5p, the putative rRNA pseudouridine synthase. Genes Dev. 12:527-537. Watkins, N. J., A. Gottschalk, G. Neubauer, B. Kastner, P. Fabrizio, M. Mann, and R. Luhrmann. 1998. Cbf5p, a potential pseudouridine synthase, and Nhp2p, a putative RNA-binding protein, are present together with Gar1p in all H BOX/ACA-motif snoRNPs and constitute a common bipartite structure. RNA 4:1549-1568. Henras, A., Y. Henry, C. Bousquet-Antonelli, J. Noaillac-Depeyre, J. P. Gelugne, and M. Caizergues-Ferrer. 1998. Nhp2p and Nop10p are essential for the function of H/ACA snoRNPs. EMBO J. 17:7078-7090. Schattner, P., W. A. Decatur, C. A. Davis, M. Ares, Jr., M. J. Fournier, and T. M. Lowe. 2004. Genome-wide searching for pseudouridylation guide snoRNAs: analysis of the Saccharomyces cerevisiae genome. Nucleic Acids Res. 32:4281-4296. Torchet, C., G. Badis, F. Devaux, G. Costanzo, M. Werner, and A. Jacquier. 2005. The complete set of H/ACA snoRNAs that guide rRNA pseudouridylations in Saccharomyces cerevisiae. RNA 11:928-938.
