ALTERNATE NAMES: None reported

LENGTH: 245 nts

PROCESS: Pseudouridine formation in rRNA, Cleavage

25S rRNA: Y2923

Guide Domains
  Click number to see the target site in the 2D structure: 25S-2923
Click number to see the approximate location of the modification site in the ribosome: 25S-2923




Click here and see that yeast and humans have corresponding modifications and guide snoRNAs.

Click here and see that yeast and Arabidopsis have corresponding modifications and guide snoRNAs.

Schizosaccharomyces pombe has an orthologous snoRNA.
Click here to examine conservation of the snoRNA in fungal genomes using BLAST.


   1 aacgcaaauu uaacagccau ucguaacacg uacaguaucu cgucgagguu gaaccccucc
  61 ggggcguucu ugacccauga agaaugugga uugguguugc aauauauuua uuauuggcuu
 121 ggacuggaga acaaauuuau cgaucuuggg ugcaacaguc uuucugucgu cuguuuuuua
 181 gcagaucuaa ggguuuaccu ucgugugccc ggaugaggac cguugcaagg auugauaaua
 241 caacu

Click here to view the SGD entry for this snoRNA.

Balakin, A. G., L. Smith, and M. J. Fournier. 1996. The RNA world of the nucleolus: two major families of small RNAs defined by different box elements with related functions. Cell 86:823-834.
Wise, J. A., D. Tollervey, D. Maloney, H. Swerdlow, E. J. Dunn, and C. Guthrie. 1983. Yeast contains small nuclear RNAs encoded by single copy genes. Cell 35:743-751.
Tollervey, D. 1987. A yeast small nuclear RNA is required for normal processing of pre-ribosomal RNA. EMBO J. 6:4169-4175.
Tollervey, D., and C. Guthrie. 1985. Deletion of a yeast small nuclear RNA gene impairs growth. EMBO J. 4:3873-3878.
Dandekar, T., and D. Tollervey. 1993. Identification and functional analysis of a novel yeast small nucleolar RNA. Nucleic Acids Res. 21:5386-5390.
Morrissey, J. P., and D. Tollervey. 1993. Yeast snR30 is a small nucleolar RNA required for 18S rRNA synthesis. Mol. Cell. Biol. 13:2469-2477.
Clark, M. W., M. L. Yip, J. Campbell, and J. Abelson. 1990. SSB-1 of the yeast Saccharomyces cerevisiae is a nucleolar-specific, silver-binding protein that is associated with the snR10 and snR11 small nuclear RNAs. J. Cell Biol. 111:1741-1751.
Ni, J., A. L. Tien, and M. J. Fournier. 1997. Small nucleolar RNAs direct site-specific synthesis of pseudouridine in ribosomal RNA. Cell 89:565-573.
Ganot, P., M. Caizergues-Ferrer, and T. Kiss. 1997. The family of box ACA small nucleolar RNAs is defined by an evolutionarily conserved secondary structure and ubiquitous sequence elements essential for RNA accumulation. Genes Dev. 11:941-956.
Girard, J. P., H. Lehtonen, M. Caizergues-Ferrer, F. Amalric, D. Tollervey, and B. Lapeyre. 1992. GAR1 is an essential small nucleolar RNP protein required for pre-rRNA processing in yeast. EMBO J. 11:673-682.
Bagni, C., and B. Lapeyre. 1998. Gar1p binds to the small nucleolar RNAs snR10 and snR30 in vitro through a nontypical RNA binding element. J. Biol. Chem. 273:10868-10873.
Lafontaine, D. L., C. Bousquet-Antonelli, Y. Henry, M. Caizergues-Ferrer, and D. Tollervey. 1998. The box H + ACA snoRNAs carry Cbf5p, the putative rRNA pseudouridine synthase. Genes Dev. 12:527-537.
Watkins, N. J., A. Gottschalk, G. Neubauer, B. Kastner, P. Fabrizio, M. Mann, and R. Luhrmann. 1998. Cbf5p, a potential pseudouridine synthase, and Nhp2p, a putative RNA-binding protein, are present together with Gar1p in all H BOX/ACA-motif snoRNPs and constitute a common bipartite structure. RNA 4:1549-1568.
Henras, A., Y. Henry, C. Bousquet-Antonelli, J. Noaillac-Depeyre, J. P. Gelugne, and M. Caizergues-Ferrer. 1998. Nhp2p and Nop10p are essential for the function of H/ACA snoRNPs. EMBO J. 17:7078-7090.
Torchet, C., G. Badis, F. Devaux, G. Costanzo, M. Werner, and A. Jacquier. 2005. The complete set of H/ACA snoRNAs that guide rRNA pseudouridylations in Saccharomyces cerevisiae. RNA 11:928-938.
King, T. H., B. Liu, R. R. McCully, and M. J. Fournier. 2003. Ribosome structure and activity are altered in cells lacking snoRNPs that form pseudouridines in the peptidyl transferase center. Mol. Cell 11:425-435.
Li, S. G., H. Zhou, Y. P. Luo, P. Zhang, and L. H. Qu. 2005. Identification and functional analysis of 20 Box H/ACA small nucleolar RNAs (snoRNAs) from Schizosaccharomyces pombe. J. Biol. Chem. 280:16446-16455.

Last update: October 13, 2006 6:00 AM.