ALTERNATE NAMES: RUF1
LENGTH: 550 nts
PROCESS: Pseudouridine formation in rRNA
TARGET SITE(S): 25S rRNA: Y2266
GENOMIC ORGANIZATION: Independent gene
GENE DISRUPTION PHENOTYPE: Viable
CORRESPONDENCES IN HUMANS AND PLANTS: Click here and see that yeast and humans have corresponding modifications and guide snoRNAs.
None reported in plants
PHYLOGENETIC CONSERVATION IN FUNGI: Click here to examine conservation of the snoRNA in fungal genomes using BLAST.
RNA SEQUENCE:
1 auugcacaac uuaaguuugu cgaggaucau uuuuuugaac ugaaucaugc ucuuuuuaag 61 ugcuuugaaa cccucgauga augugucaau gugcaaagau aaaccauugu ucucuguuga 121 ucagugacuu aauguuugcu uuggagaaug auauuuuccc uuuccuauau uugacuuuug 181 uucuaaaagu uauuuggaga gaaaaggcau gauugagguu gcgacuuuuu cguuuuugcu 241 uuugcaugga uaauucaucc augcacaucu cacuuuauug gaccuucaag auugguuucc 301 cauguaauuu aauuuucucu ccucuacauu uaauauguuc uauauuaauu aauaccaauu 361 gaguugugcg uacuucauug cagauauuuu accagaccug ucugaguuuu ucguucaagu 421 uugguugaaa ucggcuugag guauaugaac gugguuggga uauggagauu gggagaucaa 481 agaagcgaaa auaccugaga caguuuuuuu aaaaaagaag cuaaggaaca ugacucaaag 541 agacacauua
YEAST GENOME DATABASE ENTRY:Click here to view the SGD entry for this snoRNA.
REFERENCES: McCutcheon, J. P., and S. R. Eddy. 2003. Computational identification of non-coding RNAs in Saccharomyces cerevisiae by comparative genomics. Nucleic Acids Res. 31:4119-4128. Schattner, P., W. A. Decatur, C. A. Davis, M. Ares, Jr., M. J. Fournier, and T. M. Lowe. 2004. Genome-wide searching for pseudouridylation guide snoRNAs: analysis of the Saccharomyces cerevisiae genome. Nucleic Acids Res. 32:4281-4296. Torchet, C., G. Badis, F. Devaux, G. Costanzo, M. Werner, and A. Jacquier. 2005. The complete set of H/ACA snoRNAs that guide rRNA pseudouridylations in Saccharomyces cerevisiae. RNA 11:928-938.
