ALTERNATE NAMES: None reported
LENGTH: 351 nts
PROCESS: Pseudouridine formation in rRNA
TARGET SITE(S): 25S rRNA: Y2975
GENOMIC ORGANIZATION: Independent gene
GENE DISRUPTION PHENOTYPE: Viable
CORRESPONDENCES IN HUMANS AND PLANTS: Click here and see that yeast and humans have corresponding modifications and guide snoRNAs.
None reported in plants
PHYLOGENETIC CONSERVATION IN FUNGI: Schizosaccharomyces pombe has an orthologous snoRNA. Click here to examine conservation of the snoRNA in fungal genomes using BLAST.
RNA SEQUENCE:
1 auaacaauuu gugaugcuuu agggagccua uuguuugaug guuuauagga gccggauuug 61 ucuuuuugac uaauuuucaa ccuauucuua cuuccaaaac uguugcaaug gcucucguuu 121 uuuuuaaauu uuucucuuuu cucuucuuug gcauaguuua uuuuuaauaa auuauuucag 181 ggugagauag agaaaggaua augaguaucg uagauaaauc uucgguuccg aauaguguaa 241 cuguuggugc ugagguaauc caucuuuaaa accaucgccg uuagagguug cuucugauau 301 uuucgguuaa gcgacccaug aaauggugau gaccugaggu auggaacauc u
YEAST GENOME DATABASE ENTRY:Click here to view the SGD entry for this snoRNA.
REFERENCES: Li, S. G., H. Zhou, Y. P. Luo, P. Zhang, and L. H. Qu. 2005. Identification and functional analysis of 20 Box H/ACA small nucleolar RNAs (snoRNAs) from Schizosaccharomyces pombe. J. Biol. Chem. 280:16446-16455. Balakin, A. G., L. Smith, and M. J. Fournier. 1996. The RNA world of the nucleolus: two major families of small RNAs defined by different box elements with related functions. Cell 86:823-834. Ni, J., A. L. Tien, and M. J. Fournier. 1997. Small nucleolar RNAs direct site-specific synthesis of pseudouridine in ribosomal RNA. Cell 89:565-573. Ganot, P., M. Caizergues-Ferrer, and T. Kiss. 1997. The family of box ACA small nucleolar RNAs is defined by an evolutionarily conserved secondary structure and ubiquitous sequence elements essential for RNA accumulation. Genes Dev. 11:941-956. Lafontaine, D. L., C. Bousquet-Antonelli, Y. Henry, M. Caizergues-Ferrer, and D. Tollervey. 1998. The box H + ACA snoRNAs carry Cbf5p, the putative rRNA pseudouridine synthase. Genes Dev. 12:527-537. Watkins, N. J., A. Gottschalk, G. Neubauer, B. Kastner, P. Fabrizio, M. Mann, and R. Luhrmann. 1998. Cbf5p, a potential pseudouridine synthase, and Nhp2p, a putative RNA-binding protein, are present together with Gar1p in all H BOX/ACA-motif snoRNPs and constitute a common bipartite structure. RNA 4:1549-1568. Henras, A., Y. Henry, C. Bousquet-Antonelli, J. Noaillac-Depeyre, J. P. Gelugne, and M. Caizergues-Ferrer. 1998. Nhp2p and Nop10p are essential for the function of H/ACA snoRNPs. EMBO J. 17:7078-7090. King, T. H., B. Liu, R. R. McCully, and M. J. Fournier. 2003. Ribosome structure and activity are altered in cells lacking snoRNPs that form pseudouridines in the peptidyl transferase center. Mol. Cell 11:425-435.
