ALTERNATE NAMES: None reported
LENGTH: 182 nts
PROCESS: Pseudouridine formation in rRNA
TARGET SITE(S): 18S rRNA: Y1187
GENOMIC ORGANIZATION: Independent gene
GENE DISRUPTION PHENOTYPE: Viable
CORRESPONDENCES IN HUMANS AND PLANTS: Click here and see that yeast and humans have corresponding modifications and guide snoRNAs.
None reported in plants
PHYLOGENETIC CONSERVATION IN FUNGI: Schizosaccharomyces pombe has an orthologous snoRNA.
Click here to examine conservation of the snoRNA in fungal genomes using BLAST.
RNA SEQUENCE:
1 uugcccugug ccucgcucgg uuguuaauug ccaauauuac gaucuugagc uggaugacaa 61 aaaaauaaaa auacauuuua uuuuuuugau caacgggcua aaacaauuag acuucuuuga 121 guacgaggau aucgcuauuu uuaucucacg guaucguauu guaauuagaa cgucucauau 181 ca
YEAST GENOME DATABASE ENTRY:Click here to view the SGD entry for this snoRNA.
REFERENCES: Balakin, A. G., L. Smith, and M. J. Fournier. 1996. The RNA world of the nucleolus: two major families of small RNAs defined by different box elements with related functions. Cell 86:823-834. Samarsky, D. A., A. G. Balakin, and M. J. Fournier. 1995. Characterization of three new snRNAs from Saccharomyces cerevisiae: snR34, snR35 and snR36. Nucleic Acids Res. 23:2548-2554. Ganot, P., M. L. Bortolin, and T. Kiss. 1997. Site-specific pseudouridine formation in preribosomal RNA is guided by small nucleolar RNAs. Cell 89:799-809. Ganot, P., M. Caizergues-Ferrer, and T. Kiss. 1997. The family of box ACA small nucleolar RNAs is defined by an evolutionarily conserved secondary structure and ubiquitous sequence elements essential for RNA accumulation. Genes Dev. 11:941-956. Lafontaine, D. L., C. Bousquet-Antonelli, Y. Henry, M. Caizergues-Ferrer, and D. Tollervey. 1998. The box H + ACA snoRNAs carry Cbf5p, the putative rRNA pseudouridine synthase. Genes Dev. 12:527-537. Watkins, N. J., A. Gottschalk, G. Neubauer, B. Kastner, P. Fabrizio, M. Mann, and R. Luhrmann. 1998. Cbf5p, a potential pseudouridine synthase, and Nhp2p, a putative RNA-binding protein, are present together with Gar1p in all H BOX/ACA-motif snoRNPs and constitute a common bipartite structure. RNA 4:1549-1568. Henras, A., Y. Henry, C. Bousquet-Antonelli, J. Noaillac-Depeyre, J. P. Gelugne, and M. Caizergues-Ferrer. 1998. Nhp2p and Nop10p are essential for the function of H/ACA snoRNPs. EMBO J. 17:7078-7090. Li, S. G., H. Zhou, Y. P. Luo, P. Zhang, and L. H. Qu. 2005. Identification and functional analysis of 20 Box H/ACA small nucleolar RNAs (snoRNAs) from Schizosaccharomyces pombe. J. Biol. Chem. 280:16446-16455.
