ALTERNATE NAMES: U17
LENGTH: 608 nts
PROCESS: Cleavage of rRNA
TARGET SITE(S): None reported
GENOMIC ORGANIZATION: Independent gene
GENE DISRUPTION PHENOTYPE: Lethal
ORTHOLOGS IN HUMANS AND PLANTS: Click here and see that yeast and humans have an orthologous U17/snR30 snoRNA.
Click here and see that yeast and Arabidopsis have corresponding modifications and guide snoRNAs.
PHYLOGENETIC CONSERVATION IN FUNGI: Click here to examine conservation of the snoRNA in fungal genomes using BLAST.
RNA SEQUENCE:
1 aaccauaguc ucgugcuagu ucgguacuau acagggaagg gaagucacuc gcauacgugu 61 gugugcauuu cuugcuauug cugcuuagcu ucucuaaaac acugggcuac guuuuucaac 121 gcucgagagg cagagucuca aggagccucc aaugggccuc acguauucau cuagauggcg 181 cuucggacaa cggcaucaca uaagagaugc agcuccugac uucuccucug aucuucguga 241 ucagaguuuu gagucgucag acuacgagca guuucucuua gucguugcau cgggugcugu 301 ugccuuaagc auguguauau gggguucggg ggcuguugcc augauauaua uggaugagac 361 agaaguggcc ccguugacga guuuaacuua gauuaaguag gacgcaugau cuugagcucu 421 uuuccuauac uuuguccuau ggccagcuuu cuccuuauua cgaagagauu gcgggaugug 481 ggugcagagu gggaaaaucu gaguucgguc aucuuuguug uucguccuac cgcaguauau 541 uccuaaacac uaugaaauga cccuaguugg uccaugauca uuuggguaaa accauacugc 601 agacaucu
YEAST GENOME DATABASE ENTRY:Click here to view the SGD entry for this snoRNA.
REFERENCES: Balakin, A. G., L. Smith, and M. J. Fournier. 1996. The RNA world of the nucleolus: two major families of small RNAs defined by different box elements with related functions. Cell 86:823-834. Bally, M., J. Hughes, and G. Cesareni. 1988. SnR30: a new, essential small nuclear RNA from Saccharomyces cerevisiae. Nucleic Acids Res. 16:5291-5303. Morrissey, J. P., and D. Tollervey. 1993. Yeast snR30 is a small nucleolar RNA required for 18S rRNA synthesis. Mol. Cell. Biol. 13:2469-2477. Girard, J. P., H. Lehtonen, M. Caizergues-Ferrer, F. Amalric, D. Tollervey, and B. Lapeyre. 1992. GAR1 is an essential small nucleolar RNP protein required for pre-rRNA processing in yeast. EMBO J. 11:673-682. Ganot, P., M. Caizergues-Ferrer, and T. Kiss. 1997. The family of box ACA small nucleolar RNAs is defined by an evolutionarily conserved secondary structure and ubiquitous sequence elements essential for RNA accumulation. Genes Dev. 11:941-956. Bagni, C., and B. Lapeyre. 1998. Gar1p binds to the small nucleolar RNAs snR10 and snR30 in vitro through a nontypical RNA binding element. J. Biol. Chem. 273:10868-10873. Lafontaine, D. L., C. Bousquet-Antonelli, Y. Henry, M. Caizergues-Ferrer, and D. Tollervey. 1998. The box H + ACA snoRNAs carry Cbf5p, the putative rRNA pseudouridine synthase. Genes Dev. 12:527-537. Watkins, N. J., A. Gottschalk, G. Neubauer, B. Kastner, P. Fabrizio, M. Mann, and R. Luhrmann. 1998. Cbf5p, a potential pseudouridine synthase, and Nhp2p, a putative RNA-binding protein, are present together with Gar1p in all H BOX/ACA-motif snoRNPs and constitute a common bipartite structure. RNA 4:1549-1568. Henras, A., Y. Henry, C. Bousquet-Antonelli, J. Noaillac-Depeyre, J. P. Gelugne, and M. Caizergues-Ferrer. 1998. Nhp2p and Nop10p are essential for the function of H/ACA snoRNPs. EMBO J. 17:7078-7090. Atzorn, V., P. Fragapane, and T. Kiss. 2004. U17/snR30 is a ubiquitous snoRNA with two conserved sequence motifs essential for 18S rRNA production. Mol. Cell. Biol. 24:1769-1778. Eliceiri, G. L. 2006. The vertebrate E1/U17 small nucleolar ribonucleoprotein particle. J. Cell. Biochem. 98:486-495. Fayet-Lebaron, E., V. Atzorn, Y. Henry Y, and T. Kiss. 2009. 18S rRNA processing requires base pairings of snR30 H/ACA snoRNA to eukaryote-specific 18S sequences. EMBO J. XX:XXX-XXX. advance online publication 26 March 2009
