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Savinov S.N. and Heuck A.P.
Interaction of cholesterol with Perfringolysin O: what have we learned from functional analysis?

Toxins 2017
in press.

Johnson B.B., Breña M., Anguita J., and Heuck A.P.
Mechanistic Insights into the Cholesterol-dependent Binding of Perfringolysin O-based Probes and Cell Membranes

Scientific Reports 2017, 7, Article number: 13793
[Springer Nature].

Vermeulen A.J., Tang Y., Heuck A.P.
Translocation of Toxins by Gram Negative Pathogens Using the Type III Secretion System

in Toxinology. Microbial Toxins.Gopalakrishnakone, P., Alape-Girón, A., Stiles, B., Dubreuil, J.D., Mandal, M. (Eds.)
in press.

Romano F.B., Tang Y., Rossi K.C., Monopoli K.R., Ross J.L., Heuck A.P.
Type 3 Secretion translocators spontaneously assemble a hexadecameric transmembrane complex

J. Biol. Chem. 2016 291: 6304-6315.
[JBC Online]

Kim C., Patel P., Gouvin L.M., Brown M.L., Khalil A., Henchey E.M., Heuck A.P., Yadava N.*
Comparative Analysis of the Mitochondrial Physiology of Pancreatic β Cells.

Bioenergetics 2014 3(1): 110.
[Bioenergetics: Open Access]

The Journal of Biological Chemistry
The Journal of Biological Chemistry

Fahie M., Romano F.B., Chisholm C., Heuck A.P., Zbinden M., and Chen M.*
A Non-classical Assembly Pathway of Escherichia coli Pore-forming Toxin Cytolysin A.

J. Biol. Chem. 2013 288(43): pp 31042-31051.
[JBC Online]

The Journal of Biological Chemistry

Raghava S., Giorda K. M., Romano F. B., Heuck A. P., & Hebert D. N.*
SV40 late protein VP4 forms toroidal pores to disrupt membranes for viral release.

Biochemistry 2013 52(22), pp 3939-48
[Biochemistry online]

The Journal of Biological Chemistry

Divakaruni AS, Wiley SE, Rogers GW, Andreyev AY, Petrosyan S, Loviscach M, Wall EA, Yadava N, Heuck AP, Ferrick DA, Henry RR, McDonald WG, Colca JR, Simon MI, Ciaraldi TP, Murphy AN.*
Thiazolidinediones are acute, specific inhibitors of the mitochondrial pyruvate carrier.

Proc Natl Acad Sci U S A. 2013 110(14), pp 5422-27.
[PNAS online]

The Journal of Biological Chemistry

Pocognoni C.A., M.S., De Blas G.A., Heuck A.P., Belmonte, S. A., Mayorga, L.S.*
Perfringolysin O as a useful tool to study human sperm physiology.

Fertility and Sterility 2013, 99 (1), pp 99-106
[F&S online]

The Journal of Biological Chemistry

Johnson B.B., Moe P.C., Wang Y.D., Rossi K., Trigatti B.L., and Heuck A.P.
Modifications in Perfringolysin O domain 4 alter the threshold of cholesterol concentration required for binding.

Biochemistry 2012, 51 (16), pp 3373-3382

[Biochemistry online]

The Journal of Biological Chemistry

Luthra A., Zhu G., Desrosiers D. C., Eggers C. H., Mulay V. , Anand A., McArthur F. A., Romano F. B., Caimano M. J., Heuck A. P., Malkowski M. G. , and Radolf J.D.*
The transition from closed to open conformation of Treponema pallidum outer membrane-associated lipoprotein TP0453 involves membrane sensing and integration by two amphipathic helices.

J. Biol. Chem. 2011, 286(48), 41656-68. Epub Sep 29.


[JBC online]

The Journal of Biological Chemistry

Romano F. B.,; Rossi, K.; Sava, C.G.; Holzenburg, A.; Clerico, E.M.; Heuck A. P.
Efficient isolation of Pseudomonas aeruginosa type III secretion translocators and assembly of heteromeric transmembrane pores in model membranes.

Biochemistry 2011.50 (33), pp 7117–7131. Free article online.

[Biochemistry online]

The Journal of Biological Chemistry

Raghava S., Giorda K. M., Romano F. B., Heuck A. P., & Hebert* D. N.
The SV40 late protein VP4 is a viroporin that forms pores to disrupt membranes for viral release.
PLoS Pathogens 2011. 7 (6), e1002116.


[PLoS Pathogens online]

The Journal of Biological Chemistry

Moe P.C. & Heuck A.P.
Phospholipid Hydrolysis Caused by Clostridium perfringens alpha-toxin Facilitates the Targeting of Perfringolysin O to Membrane Bilayers.

Biochemistry 2010 49 (44) pp 9498-9507.

[Biochemistry online]

The Journal of Biological Chemistry

Heuck A.P.*, Moe P.C., Johnson, B.B. 2010
The cholesterol-dependent cytolysins family of Gram-positive bacterial toxins. (pdf)
Subcellular Biochemistry 51:551-77.


The original publication will be available at: [Springer Web page] [PubMed]

The Journal of Biological Chemistry

Flanagan J.J., Tweten R.K., Johnson A.E., Heuck A.P. 2009
Cholesterol Exposure at the Membrane Surface Is Necessary and Sufficient to Trigger Perfringolysin O Binding
Biochemistry, 2009 May 12;48(18):3977-87.

[Biochemistry Online]

The Journal of Biological Chemistry

Bowden M.G., Heuck A.P., Ponnuraj K., Kolosova E., Choe D., Gurusiddappa S., Narayana S.V.L. , Johnson A.E., and Hook M. 2008
Evidence for the "Dock, Lock and Latch" ligand binding mechanism of the staphylococcal MSCRAMM SdrG.
The Journal of Biological Chemistry, 283, (1), 638-647.

[JBC Online]

The Journal of Biological Chemistry

Heuck A.P.*, Savva C.G., Holzenburg A., Johnson A.E. 2007
Conformational changes that effect oligomerization and initiate pore formation are triggered throughout perfringolysin O upon binding to cholesterol
The Journal of Biological Chemistry, 282(31): 22629-37



[JBC Online]

Protein-Lipid Interactions
Heuck A.P., Johnson A.E. 2005
Membrane recognition and pore formation by bacterial pore-forming toxins
In Protein-lipid interactions, L. Tamm Editor





[Wiley-VHC]
The Journal of Biological Chemistry
Heuck A.P., Tweten R.K., Johnson A.E. 2003
Assembly and topography of the prepore complex in cholesterol-dependent cytolysins
The Journal of Biological Chemistry, 278(33):31218-25




[Pubmed]
Ramachandran R., Heuck A.P., Tweten R.K., Johnson A.E. 2002
Structural insights into the membrane-anchoring mechanism of a cholesterol-dependent cytolysin
Nature Structural Biology, 9(11):823-7





[PubMed]
The journal of Biochemistry
Hotze E.M., Heuck A.P., Czajkowsky D.M., Shao Z., Johnson A.E., Tweten R.K. 2002
Monomer-monomer interactions drive the prepore to pore conversion of a beta-barrel-forming cholesterol-dependent cytolysin
The Journal of Biological Chemistry, 277(13):11597-605

 

Cell Biochemistry and Biiophysics
Heuck A.P., Johnson A.E. 2002
Pore-forming protein structure analysis in membranes using multiple independent fluorescence techniques
Cell Biochemistry and Biophysics, 36(1):89-101



[PubMed]
Biochemistry
Heuck A.P., Tweten R.K., Johnson A.E. 2001
Beta-barrel pore-forming toxins:intriguing dimorphic proteins
Biochemistry, 40(31):9065-73

 

Molecular Cell
Heuck A.P. , Hotze E.M., Tweten R.K., Johnson A.E. 2000
Mechanism of membrane insertion of a multimeric beta-barrel protein: perfringolysin O creates a pore using ordered and coupled conformational changes
Molecular Cell, 6(5):1233-42


[PubMed]
Cell
Shatursky O., Heuck A.P., Shepard L.A., Rossjohn J., Parker M.W., Johnson A.E., Tweten R.K. 1999
The mechanism of membrane insertion for a cholesterol-dependent cytolysin: a novel paradigm for pore-forming toxins
Cell, 99(3):293-9

[PubMed]
Shepard L.A., Heuck A.P., Hamman B.D., Rossjohn J., Parker M.W., Ryan K.R., Johnson A.E., Tweten R.K. 1998
Identification of a membrane-spanning domain of the thiol-activated pore-forming toxin Clostridium perfringens perfringolysin O: an alpha-helical to beta-sheet transition identified by fluorescence spectroscopy
Biochemistry, 37(41):14563-74

[PubMed]
Biochemical and Biophysical Methods
Heuck A.P., Wolosiuk R.A. 1997
Fluoresceinthiocarbamyl-insulin: a potential analytical tool for the assay of disulfide bond reduction
Journal of Biochemical and Biophysical Methods, 34(3):213-25




[PubMed]
Analytical Chemistry
Heuck A.P., Wolosiuk R.A. 1997
Di-fluoresceinthiocarbamyl-insulin: a fluorescent substrate for the assay of protein disulfide oxidoreductase activity
Analytical Biochemistry, 248(1):94-101



PubMed]

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