ALTERNATE NAMES: snR128
LENGTH: 126 nts
PROCESS: 2'-O-methylation of rRNA, cleavage of rRNA - conserved in Eukaryotes
TARGET SITE AND PAIRING(S): 18S rRNA: Cm414 - verified by D.P.P. (unpublished).
GENOMIC ORGANIZATION: Polycistronic gene
GENE DISRUPTION PHENOTYPE: Lethal
CORRESPONDENCES IN HUMANS AND PLANTS: Click here and see that yeast and humans have corresponding modifications and guide snoRNAs. Click here and see that yeast and Arabidopsis have corresponding modifications and guide snoRNAs.
PHYLOGENETIC CONSERVATION IN FUNGI: Click here to examine conservation of the snoRNA in fungal genomes using BLAST.
RNA SEQUENCE:
1 ucacggugau gaaagacugg uuccuuaaca uucgcaguuu ccacgguagg aguacgcuua 61 cgaacccauc guuaguacuc ucggugaccg cucuucuuua gagaccuucc uaggaugucu 121 gaguga
YEAST GENOME DATABASE ENTRY:Click here to view the SGD entry for this snoRNA.
REFERENCES: Zagorski, J., D. Tollervey, and M. J. Fournier. 1988. Characterization of an SNR gene locus in Saccharomyces cerevisiae that specifies both indispensable and essential small nuclear RNAs. Mol. Cell. Biol. 8:3282-3290. Li, H. D., J. Zagorski, and M. J. Fournier. 1990. Depletion of U14 small nuclear RNA (snR128) disrupts production of 18S rRNA in Saccharomyces cerevisiae. Mol. Cell. Biol. 10:1145-1152. Kiss-Laszlo, Z., Y. Henry, J. P. Bachellerie, M. Caizergues-Ferrer, and T. Kiss. 1996. Site-specific ribose methylation of preribosomal RNA: a novel function for small nucleolar RNAs. Cell 85:1077-1088. Balakin, A. G., L. Smith, and M. J. Fournier. 1996. The RNA world of the nucleolus: two major families of small RNAs defined by different box elements with related functions. Cell 86:823-834. Ganot, P., M. Caizergues-Ferrer, and T. Kiss. 1997. The family of box ACA small nucleolar RNAs is defined by an evolutionarily conserved secondary structure and ubiquitous sequence elements essential for RNA accumulation. Genes Dev. 11:941-956. Dunbar, D. A., and S. J. Baserga. 1998. The U14 snoRNA is required for 2'-O-methylation of the pre-18S rRNA in Xenopus oocytes. RNA 4:195-204. Wu, P., J. S. Brockenbrough, A. C. Metcalfe, S. Chen, and J. P. Aris. 1998. Nop5p is a small nucleolar ribonucleoprotein component required for pre-18S rRNA processing in yeast. J. Biol. Chem. 273:16453-16463. Samarsky, D. A., G. S. Schneider, and M. J. Fournier. 1996. An essential domain in Saccharomyces cerevisiae U14 snoRNA is absent in vertebrates, but conserved in other yeasts. Nucleic Acids Res. 24:2059-2066. Liang, W. Q., J. A. Clark, and M. J. Fournier. 1997. The rRNA-processing function of the yeast U14 small nucleolar RNA can be rescued by a conserved RNA helicase-like protein. Mol. Cell. Biol. 17:4124-4132. Chanfreau, G., G. Rotondo, P. Legrain, and A. Jacquier. 1998. Processing of a dicistronic small nucleolar RNA precursor by the RNA endonuclease Rnt1. EMBO J. 17:3726-3737. Kos, M., and D. Tollervey. 2005. The putative RNA helicase Dbp4p is required for release of the U14 snoRNA from preribosomes in Saccharomyces cerevisiae. Mol. Cell 20:53-64. Chen, W., J. Bucaria, D. A. Band, A. Sutton, and R. Sternglanz. 2003. Enp1, a yeast protein associated with U3 and U14 snoRNAs, is required for pre-rRNA processing and 40S subunit synthesis. Nucleic Acids Res. 31:690-699. Piekna-Przybylska, D., and M. J. Fournier. (unpublished). In regards to 2'-O-methylation of rRNA.
