Luthra A., Zhu G., Desrosiers D. C., Eggers C. H., Mulay V. , Anand A., McArthur F. A., Romano F. B., Caimano M. J., Heuck A. P., Malkowski M. G. , and Radolf J.D.*
The transition from closed to open conformation of Treponema pallidum outer membrane-associated lipoprotein TP0453 involves membrane sensing and integration by two amphipathic helices J. Biol. Chem. 2011, 286(48), 41656-68. Epub Sep 29.

[JBC online]

Romano F. B.,; Rossi, K.; Sava, C.G.; Holzenburg, A.; Clerico, E.M.; Heuck A. P.
Efficient isolation of Pseudomonas aeruginosa type III secretion translocators and assembly of heteromeric transmembrane pores in model membranes Biochemistry 2011.50 (33), pp 7117–7131 Jul 19. [Epub ahead of print].

[Biochemistry online]

Raghava S., Giorda K. M., Romano F. B., Heuck A. P., & Hebert* D. N.
The SV40 late protein VP4 is a viroporin that forms pores to disrupt membranes for viral release
PLoS Pathogens 2011. 7 (6), e1002116.

[PLoS Pathogens online]

Moe P.C. & Heuck A.P.
Phospholipid Hydrolysis Caused by Clostridium perfringens alpha-toxin Facilitates the Targeting of Perfringolysin O to Membrane Bilayers
Biochemistry 2010 49 (44) pp 9498-9507.

[Biochemistry online]

Heuck A.P.*, Moe P.C., Johnson, B.B. 2010
The cholesterol-dependent cytolysins family of Gram-positive bacterial toxins. (pdf)
Subcellular Biochemistry 51:551-77.

The original publication will be available at: [Springer Web page] [PubMed]

Flanagan J.J., Tweten R.K., Johnson A.E., Heuck A.P.* 2009
Cholesterol Exposure at the Membrane Surface Is Necessary and Sufficient to Trigger Perfringolysin O Binding
Biochemistry, 2009 May 12;48(18):3977-87.


[Biochemistry Online]

Bowden M.G., Heuck A.P., Ponnuraj K., Kolosova E., Choe D., Gurusiddappa S., Narayana S.V.L. , Johnson A.E., and Hook M. 2008
Evidence for the "Dock, Lock and Latch" ligand binding mechanism of the staphylococcal MSCRAMM SdrG.
The Journal of Biological Chemistry, 283, (1), 638-647.


[JBC Online]

Heuck A.P.*, Savva C.G., Holzenburg A., Johnson A.E. 2007
Conformational changes that effect oligomerization and initiate pore formation are triggered throughout perfringolysin O upon binding to cholesterol
The Journal of Biological Chemistry, 282(31): 22629-37



[JBC Online]

Heuck A.P., Johnson A.E. 2005
Membrane recognition and pore formation by bacterial pore-forming toxins
In Protein-lipid interactions, L. Tamm Editor





[Wiley-VHC]

Heuck A.P., Tweten R.K., Johnson A.E. 2003
Assembly and topography of the prepore complex in cholesterol-dependent cytolysins
The Journal of Biological Chemistry, 278(33):31218-25




[Pubmed]

Ramachandran R., Heuck A.P., Tweten R.K., Johnson A.E. 2002
Structural insights into the membrane-anchoring mechanism of a cholesterol-dependent cytolysin
Nature Structural Biology, 9(11):823-7





[PubMed]

Hotze E.M., Heuck A.P., Czajkowsky D.M., Shao Z., Johnson A.E., Tweten R.K. 2002
Monomer-monomer interactions drive the prepore to pore conversion of a beta-barrel-forming cholesterol-dependent cytolysin
The Journal of Biological Chemistry, 277(13):11597-605

[PubMed]

Heuck A.P., Johnson A.E. 2002
Pore-forming protein structure analysis in membranes using multiple independent fluorescence techniques
Cell Biochemistry and Biophysics, 36(1):89-101



[PubMed]

Heuck A.P., Tweten R.K., Johnson A.E. 2001
Beta-barrel pore-forming toxins:intriguing dimorphic proteins
Biochemistry, 40(31):9065-73

Heuck A.P. , Hotze E.M., Tweten R.K., Johnson A.E. 2000
Mechanism of membrane insertion of a multimeric beta-barrel protein: perfringolysin O creates a pore using ordered and coupled conformational changes
Molecular Cell, 6(5):1233-42


[PubMed]

Shatursky O., Heuck A.P., Shepard L.A., Rossjohn J., Parker M.W., Johnson A.E., Tweten R.K. 1999
The mechanism of membrane insertion for a cholesterol-dependent cytolysin: a novel paradigm for pore-forming toxins
Cell, 99(3):293-9

[PubMed]

Shepard L.A., Heuck A.P., Hamman B.D., Rossjohn J., Parker M.W., Ryan K.R., Johnson A.E., Tweten R.K. 1998
Identification of a membrane-spanning domain of the thiol-activated pore-forming toxin Clostridium perfringens perfringolysin O: an alpha-helical to beta-sheet transition identified by fluorescence spectroscopy
Biochemistry, 37(41):14563-74

[PubMed]

Heuck A.P., Wolosiuk R.A. 1997
Fluoresceinthiocarbamyl-insulin: a potential analytical tool for the assay of disulfide bond reduction
Journal of Biochemical and Biophysical Methods, 34(3):213-25




[PubMed]

Heuck A.P., Wolosiuk R.A. 1997
Di-fluoresceinthiocarbamyl-insulin: a fluorescent substrate for the assay of protein disulfide oxidoreductase activity
Analytical Biochemistry, 248(1):94-101



PubMed]