To see the PDF files for the presentations click on the figure located on the left.
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Wed Dec 12
Lecture 13
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Announcements:
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Presenters: Rajarshi Ghosh and Yong Tong
Discussion Leaders : Sravanti Vaidya, Oscar Miranda, and Ankit Vahia
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Paper:
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Protein-protein Förster resonance energy transfer analysis of nucleosome core particles containing H2A and H2A.Z. Hoch D.A., Stratton J.J., Gloss L.M. J Mol Biol. 2007 371(4):971-88 PDF
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Wed Dec 5
Lecture 12
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Announcements:
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Presenters: Sravanti Vaidya and Siriporn Jiwpanich
Discussion Leaders : Will Boston-Howes,Shiv Redhu, and Nat Clarck
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Paper:
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Direct sugar binding to LacY measured by resonance energy transfer. Smirnova I.N., Kasho V.N., Kaback H.R.Biochemistry. 2006 Dec 26;45(51):15279-87. PDF
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Wed Nov 28
Lecture 11
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Announcements:
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Presenters: Oscar Miranda and Partha Ghosh
Discussion Leaders : Hilliary Hogdon, Rajarshi Ghosh, and Abigail Guce
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Paper:
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Fluorogenic probes for monitoring peptide binding to class II MHC proteins in living cells.. Venkatraman P., Nguyen T.T., Sainlos M., Bilsel O., Chitta S., Imperiali B., Stern L.J. Nat Chem Biol. 2007 Apr;3(4):201-2. PDF
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Wed Nov 21
Lecture 10
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Announcements:
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Nov 14 is Monday schedule. No class. This presentation will take place on Nov 21.
Presenters: Seena Koshy and Aruni K.
Discussion Leaders : Shiv Redhu, Sarit Agasti, and Siriporn Jiwpanich
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Paper:
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Sensitive Fluorescence Polarization Technique for Rapid Screening of alpha-Synuclein Oligomerization/Fibrillization Inhibitors.. Luk KC, Hyde EG, Trojanowski JQ, Lee VM. Biochemistry. 2007 46(44):12522-9 PDF
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Wed Nov 7
Lecture 9
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Announcements:
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Presenters: William Boston-Howes and Fabian Romano
Discussion Leaders : Jiang Hong, Mrinmoy De, Anna Alves
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Paper:
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Tilted, Extended, and Lying in Wait: The Membrane-Bound Topology of Residues Lys-381-Ser-405 of the Colicin E1 Channel Domain. Wei, Z.; White, D.; Wang, J.; Musse, A. A.; Merrill, A. R. Biochemistry; 2007; 46(20); 6074-6085. PDF
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Wed Oct 31
Lecture 8
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Announcements:
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Presenters: Rob Smock, Nat Clarck
Discussion Leaders : Qinghua Wang, Fabian Romano, Rahgunath Roy
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Paper:
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Real-time fluorescence detection of ERAD substrate retrotranslocation in a mammalian in vitro system. Wahlman J, DeMartino GN, Skach WR, Bulleid NJ, Brodsky JL, Johnson AE. Cell. 2007 ;129:943-55. PDF
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Wed Oct 24
Lecture 7
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Announcements:
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Presenters: Ankit Vahia, Shiv Redhu
Discussion Leaders : Hare Sahoo, Vanessa Mendoza, Witold Witowski
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Paper:
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CD4 and CCR5 Constitutively Interact at the Plasma Membrane of Living Cells A CONFOCAL FLUORESCENCE RESONANCE ENERGY TRANSFER-BASED APPROACH G. Gaibelet, T. Planchenault, S. Mazeres, F. Dumas, F. Arenzana-Seisdedos, A. Lopez, B. Lagane, and F. Bachelerie. J. Biol. Chem., Vol. 281, Issue 49, 37921-37929 PDF
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Wed Oct 17
Lecture 6
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Announcements:
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Presenters: Kristen Huber, Witold Witowski
Discussion Leaders : Myoung-Hwan Park, Partha Ghosh
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Paper:
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A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Kogure, T., Karasawa, S., Araki, T., Saito, K., Kinjo, M. and Miyawaki, A. (2006) Nat. Biotechnol., 24, 577-581. PDF
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Wed Oct 10
Lecture 5
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Announcements:
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Plase, for Oct 17 and Oct 24 send papers for approval to Prof. Craig Martin.
Presenter: Rahgunath Roy
Discussion Leaders : Aruni K., Sravanti Vaidya, Rajarshi Ghosh
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Paper:
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Label-free continuous enzyme assays with macrocycle-fluorescent dye complexes.
Hennig et al. Nat. Methods. 2007 4:629-632 PDF
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Wed Oct 3
Lecture 4
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Announcements:
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Presenters: Myoung-Hwan Park, Mrinmoy De
Discussion Leaders : Seena Koshy, Nat Clark, Rob Smock
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Paper:
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FRET analysis of protein conformational change through position-specific incorporation of fluorescent amino acids.
Kajihara et al. 2006 Nat. Methods. 2006 3:923-9 PDF Suplemental Info PDF
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Wed Sep 26
Lecture 3
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Announcements:
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Presenters: Sarit Agasti, Abigail Guce, Vanessa Mendoza
Discussion Leaders : Oscar Miranda, Rahgunath Roy, Will Boston-Howes
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Paper:
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Proteolytic activity monitored by fluorescence resonance energy transfer through quantum-dot-peptide conjugates.
Medintz et al. 2006 Nat Mater. 5, 581-9. PDF
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Wed Sep 19
Lecture 2
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Announcements:
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Presenters: Jiang Hong, Harekrushna Sahoo, Qinghua Wang
Discussion Leaders : Witold Witowski, Yong Tong, Fabian Romano
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Paper:
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Specific and Stable Fluorescence Labeling of Histidine-Tagged Proteins for Dissecting Multi-Protein Complex Formation.
Lata et al. 2006 JACS 128, 2365-2372. PDF
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Wed Sep 12
Lecture 1
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Announcements:
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Welcome to the "Fluorescence Approaches in Biochemistry" Journal Club.
On Wednesday we will give some outlines on how we expect you to present the papers and we will make the final schedule for the presentations. We will also assign and schedule the discussion groups. These groups are not going to be the same as the ones you formed for the presentations. Our goal is to have 3 people with different background (i.e. different labs), so they can analyze and discuss the papers from a different perspective.
Papers selected for presentations should be submitted to the instructors 2 weeks in advance for approval.
Randomly, a one question quiz on the current paper may be given at the end of the presentation to be answered for all students. Be prepare.
A Blog was created to stimulate the discussion on general topics related to the Journal Club or Fluorescence Spectroscopy in general. Have you seen an interesting web page? , Have you found an interesting course in fluorescence?, Have you learned about a new fellowship? Meetings? , etc. Well, you can share that with us in the Blog. |
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Lecture:
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How to prepare a good paper presentation: some common mistakes when presenting data. (A. Heuck) |
Other activities:
Final Group Assignments
Organize Presentation Schedule |
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